Photosensitized inactivation of Plasmodium falciparum in human red cells by phthalocyanines
Identifieur interne : 002A26 ( Main/Exploration ); précédent : 002A25; suivant : 002A27Photosensitized inactivation of Plasmodium falciparum in human red cells by phthalocyanines
Auteurs : S. Lustigman [États-Unis] ; E. Ben-HurSource :
- Transfusion [ 0041-1132 ] ; 1996-06.
English descriptors
- Teeft :
- Aluminum phthalocyanine derivatives, Associate member, Blood cell, Blood sterilization, Cell suspensions, Chemotherapeutic agent, Crisis forms, Fakiparum clone, Falciparum, Falciparum malaria, Hematocrit, Human immunodeficiency virus, Inactivation, Lethal dose, Light dose, Light exposure, Malaria, Malaria parasites, Multiple forms, Parasite, Parasitemia, Parasitized, Parasitized rbcs, Photobiol, Photochem, Photochem photobiol, Photochemical approaches, Photodynamic, Photodynamic inactivation, Photodynamic therapy, Photodynamic treatment, Phthalocyanine, Phthalocyanine photosensitization, Phthalocyanines, Plasmodium, Plasmodium falciparum, Rbc, Ring stage, Rockefeller university, Single experiment, Stock solutions, Thin film, Transfusion, Trophozoite stage, Vesicular stomatitis virus, Vesicular stomatitis virus inactivation, Viral safety, Virus inactivation, York blood center.
Abstract
BACKGROUND: Photodynamic treatment of red cell concentrate with phthalocyanines and red light inactivates lipid‐enveloped viruses such as vesicular stomatitis virus and human immunodeficiency virus. This procedure is evaluated for its ability to enhance the viral safety of red cell concentrate for transfusion. It is of interest to study whether photodynamic treatment could also inactivate parasites in blood (e.g., Plasmodium falciparum). STUDY DESIGN AND METHODS: Red cells parasitized by P. falciparum were treated with phthalocyanines and red light and then cultured in vitro for 48 hours. The percentage of parasitemia was then estimated by microscopic examination of the red cells. RESULTS: Of the phthalocyanines studied, the one that proved to be the most effective was HOSiPcOSi(CH3)2(CH2)3N(CH3)2 (Pc4). The extent of parasite inactivation increased with light dose and decreased with an increase in hematocrit. At a hematocrit of 60 percent and 2 microM Pc 4,>or= 3 log10 kill occurred at a light dose of 60 J per cm2. This is a lower dose than is required for>or= 6 log10 of vesicular stomatitis virus inactivation (90 J/cm2). CONCLUSION: Photodynamic treatment with Pc 4 could make red cell concentrate not only virally safe for transfusion but also safe with respect to transmitting malaria.
Url:
DOI: 10.1046/j.1537-2995.1996.36696269514.x
Affiliations:
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Lustigman</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Correspondence address: Sara Lustigman, PhD, Associate Member, New York Blood Center, 310 East 67th Street, New York</wicri:cityArea></affiliation></author><author><name sortKey="Ben Ur, E" sort="Ben Ur, E" uniqKey="Ben Ur E" first="E." last="Ben-Hur">E. Ben-Hur</name></author></analytic><monogr></monogr><series><title level="j" type="main">Transfusion</title><title level="j" type="alt">TRANSFUSION</title><idno type="ISSN">0041-1132</idno><idno type="eISSN">1537-2995</idno><imprint><biblScope unit="vol">36</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="543">543</biblScope><biblScope unit="page" to="546">546</biblScope><biblScope unit="page-count">4</biblScope><publisher>Blackwell Science Ltd</publisher><pubPlace>Edinburgh, UK</pubPlace><date type="published" when="1996-06">1996-06</date></imprint><idno type="ISSN">0041-1132</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0041-1132</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Aluminum phthalocyanine derivatives</term><term>Associate member</term><term>Blood cell</term><term>Blood sterilization</term><term>Cell suspensions</term><term>Chemotherapeutic agent</term><term>Crisis forms</term><term>Fakiparum clone</term><term>Falciparum</term><term>Falciparum malaria</term><term>Hematocrit</term><term>Human immunodeficiency virus</term><term>Inactivation</term><term>Lethal dose</term><term>Light dose</term><term>Light exposure</term><term>Malaria</term><term>Malaria parasites</term><term>Multiple forms</term><term>Parasite</term><term>Parasitemia</term><term>Parasitized</term><term>Parasitized rbcs</term><term>Photobiol</term><term>Photochem</term><term>Photochem photobiol</term><term>Photochemical approaches</term><term>Photodynamic</term><term>Photodynamic inactivation</term><term>Photodynamic therapy</term><term>Photodynamic treatment</term><term>Phthalocyanine</term><term>Phthalocyanine photosensitization</term><term>Phthalocyanines</term><term>Plasmodium</term><term>Plasmodium falciparum</term><term>Rbc</term><term>Ring stage</term><term>Rockefeller university</term><term>Single experiment</term><term>Stock solutions</term><term>Thin film</term><term>Transfusion</term><term>Trophozoite stage</term><term>Vesicular stomatitis virus</term><term>Vesicular stomatitis virus inactivation</term><term>Viral safety</term><term>Virus inactivation</term><term>York blood center</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">BACKGROUND: Photodynamic treatment of red cell concentrate with phthalocyanines and red light inactivates lipid‐enveloped viruses such as vesicular stomatitis virus and human immunodeficiency virus. This procedure is evaluated for its ability to enhance the viral safety of red cell concentrate for transfusion. It is of interest to study whether photodynamic treatment could also inactivate parasites in blood (e.g., Plasmodium falciparum). STUDY DESIGN AND METHODS: Red cells parasitized by P. falciparum were treated with phthalocyanines and red light and then cultured in vitro for 48 hours. The percentage of parasitemia was then estimated by microscopic examination of the red cells. RESULTS: Of the phthalocyanines studied, the one that proved to be the most effective was HOSiPcOSi(CH3)2(CH2)3N(CH3)2 (Pc4). The extent of parasite inactivation increased with light dose and decreased with an increase in hematocrit. At a hematocrit of 60 percent and 2 microM Pc 4,>or= 3 log10 kill occurred at a light dose of 60 J per cm2. This is a lower dose than is required for>or= 6 log10 of vesicular stomatitis virus inactivation (90 J/cm2). CONCLUSION: Photodynamic treatment with Pc 4 could make red cell concentrate not only virally safe for transfusion but also safe with respect to transmitting malaria.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region></list><tree><noCountry><name sortKey="Ben Ur, E" sort="Ben Ur, E" uniqKey="Ben Ur E" first="E." last="Ben-Hur">E. Ben-Hur</name></noCountry><country name="États-Unis"><region name="État de New York"><name sortKey="Lustigman, S" sort="Lustigman, S" uniqKey="Lustigman S" first="S." last="Lustigman">S. Lustigman</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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